primary mouse monoclonal anti-pgk1 (Thermo Fisher)
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Thermo Fisher
primary mouse monoclonal anti-pgk1
Primary Mouse Monoclonal Anti Pgk1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pgk1/anti+pgk1/pmc12222759-453-43-47
Average 90 stars, based on 1 article reviews
Primary Mouse Monoclonal Anti Pgk1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pgk1/anti+pgk1/pmc12222759-453-43-47
Average 90 stars, based on 1 article reviews
primary mouse monoclonal anti-pgk1 - by Bioz Stars,
2026-10
90/100 stars
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Centrifugation:Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. Article Snippet: Strains were grown to 0.8 OD600, followed by addition of cycloheximide (C4859, Sigma-Aldrich) to a final concentration of 100μg/ml to block protein synthesis. .. At each time point, ~8 × 107 cells were harvestedby centrifugation.Whole cell extracts wereprepared by alkaline lysis followed by precipitation with trichloroacetic acid95 and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Alkaline Lysis:Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. Article Snippet: Strains were grown to 0.8 OD600, followed by addition of cycloheximide (C4859, Sigma-Aldrich) to a final concentration of 100μg/ml to block protein synthesis. .. At each time point, ~8 × 107 cells were harvestedby centrifugation.Whole cell extracts wereprepared by alkaline lysis followed by precipitation with trichloroacetic acid95 and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Western Blot:Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. Article Snippet: Strains were grown to 0.8 OD600, followed by addition of cycloheximide (C4859, Sigma-Aldrich) to a final concentration of 100μg/ml to block protein synthesis. .. At each time point, ~8 × 107 cells were harvestedby centrifugation.Whole cell extracts wereprepared by alkaline lysis followed by precipitation with trichloroacetic acid95 and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Control:Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. Article Snippet: Strains were grown to 0.8 OD600, followed by addition of cycloheximide (C4859, Sigma-Aldrich) to a final concentration of 100μg/ml to block protein synthesis. .. At each time point, ~8 × 107 cells were harvestedby centrifugation.Whole cell extracts wereprepared by alkaline lysis followed by precipitation with trichloroacetic acid95 and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), Article Title: Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons Article Snippet: .. Whole cell extracts were prepared by alkaline lysis followed by precipitation with trichloroacetic acid and separated by SDS-PAGE, followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (1:2000 dilution, 11814460001, Roche), Incubation:Article Title: Duplicated ribosomal protein paralogs promote alternative translation and drug resistance. Article Snippet: Protein samples (20 μg)were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). .. Membranes were blocked overnight at 4 °C with 5% milk in TBS-T (20mM Tris pH 7.6, 150mM NaCl, 0.1% Tween-20), then incubated overnight at 4 °C with the following primary antibodies: rabbit anti-L7 (Bethyl Laboratories, cat#A3100-741A-M, 1:1000), rabbit anti-L5 (kindly provided by Woolford lab, 1:2000), mouse anti-L3 (kindly provided by the Warner lab, 1:5000) and Blocking Assay:Article Title: Duplicated ribosomal protein paralogs promote alternative translation and drug resistance. Article Snippet: Protein samples (20 μg)were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). .. Membranes were blocked overnight at 4 °C with 5% milk in TBS-T (20mM Tris pH 7.6, 150mM NaCl, 0.1% Tween-20), then incubated overnight at 4 °C with the following primary antibodies: rabbit anti-L7 (Bethyl Laboratories, cat#A3100-741A-M, 1:1000), rabbit anti-L5 (kindly provided by Woolford lab, 1:2000), mouse anti-L3 (kindly provided by the Warner lab, 1:5000) and |
